Peptide correction of prothrombotic erythrocytes effects on platelet aggregation: 612.115.3-615.273.53

Abstract

Introduction. Hemostasis changes and disturbances of blood rheological properties play the important role in pathogenesis of many diseases. One of the actual problems of modern hematology is new drugs investigation for affecting on blood cells interaction during thrombus formation.

Aim: to compare the effect of small regulatory peptides (fragments of neurohormones) on the interaction of erythrocytes with platelets under their aggregation by adrenaline in in vitro experiments.

Materials and methods. We studied 2 peptides (C-terminal fragments of neurohormones): Pro-Arg-Gly-NH2 — fragment of vasopressin, Pro-Leu-Gly-NH2 — fragment of oxytocin. In in vitro experiments we added peptides (in concentration of 10–4–10–6 М) to a pool of platelet-rich rat plasma (PRP) or to a mixture of PRP with erythrocyte mass diluted 1:1000 by saline solution, or to washed erythrocytes and platelets and their mixture, and determined the aggregation changes under adrenalin in a final concentration of 0.02 mmol/L. Erythrocytes and platelets aggregation was recorded by aggregometer.

Results. We found that C-terminal fragment of vasopressin has a more pronounced antiplatelet effect than the oxytocin fragment, and it was found both in PRP and with the use of washed blood cells. The inhibitory effect of Pro-Arg-Gly-NH2 fragment also remained under preliminary increasing of platelet aggregation by erythrocytes. C-terminal fragment of oxytocin practically had no effect on platelet aggregation.

Conclusion. Use of small regulatory peptides helps to reduce the aggregation effect of erythrocytes, thereby improving therapy of thrombotic complications.

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