Role of glycine-containing oligopeptides in blood cells aggregation stimulated by adrenaline: 612.115.3:615.273.53

Abstract

Introduction. The changing of blood rheological properties is typical for various diseases; many of them are associated with rheology disorder and primarily with change of erythrocytes aggregation.

The aim was to compare the effect of small regulatory peptides (fragments of neurohormones) on the aggregation of erythrocytes and platelets under adrenaline action in experiments in vitro.

Materials and methods. Experiments were conducted on white outbred male rats, body weight 180–200 g. We used 2 peptides — C-terminal fragments of vasopressin (Pro-Arg-Gly-NH2) and oxytocin (Pro-Leu-Gly-NH2).

Results. It was shown that small regulatory peptides (they are products of neuropeptides proteolysis) had a significant effect on blood cells aggregation. We compared the peptides effect on blood cells aggregation stimulated by adrenaline. It was found that vasopressin fragment Pro-Arg-Gly-NH2 significantly increased both erythrocytes and platelets aggregation, while oxytocin fragment Pro-Leu-Gly-NH2 inhibited only erythrocytes aggregation without changing of platelet aggregation.

Conclusion. Investigation of phased peptides proteolysis may result in the development of new drugs for targeted correction of various disturbances. So it is of current interest to study the effects of C-terminal fragments of pituitary hormones on hemostasis.

References

1. Golubeva M. G., Grigorjeva M. Е. Effect neurohypophysial hormones and their synthetic analogs on haemostasis system. In: Progress in Neuropeptide Research. N. Y., USA: Nova Science Publishers Inc. 2007: 193–215.
2. Grigorjeva M. Е., Golubeva M. G. Oxitocin: non-classic effects and influence on haemostasis system. In: Oxitocin: Synthesis, Storage and Release Actions and Drug Forms. N. Y., USA: Nova Science Publishers Inc. 2009: 255–68.
3. Grishin E. V., Korolkova Yu. V., Tseng G. N. Biological activity of the C-terminal fragment of arginine vasopressin. Molecular mechanism of blocking the toxin scorpion potassium channel ERG-type [Biologicheskaya aktivnost’ S-koncevogo fragmenta arginin vazopressina. Molekulyarnyj mekhanizm blokirovaniya toksinom skorpiona kalievogo kanala ERG-tipa]. Vserossijskij simpozium po himii i biologii peptidov: tezisy dokl. Moskva. 2003: 30 (in Russ.).
4. Golubovich V. P., Tonkovich I. N., Figlovskiy V. A. Modeling the process of degradation of peptide compounds in the bloodstream [Modelirovanie processa degradacii peptidnyh soedinenij v rusle krovi]. Vserossijskij simpozium po himii i biologii peptidov: tezisy dokl. Moskva. 2003: 13 (in Russ.).
5. Golubeva M. G., Golubovich V. P., Melnik O. V., Martinovich V. P. Influence of neurohypophysisular C-terminal oligopeptides on platelet aggregation [Vliyanie nejrogipofizarnyh S-koncevyh oligopeptidov na agregaciyu trombocitov]. Tromboz, gemostaz i reologiya. 2007; (3): 27–31 (in Russ.).
6. Maimistova A. A., Koshelev V. B., Bulaeva S. V., Muraveva A. V. Change in aggregation and deformability of erythrocytes during activation of intracellular signaling pathways [Izmenenie agregacii i deformiruemost’ eritrocitov pri aktivacii vnutrikletochnyh signal’nyh putej]. Yaroslavskij pedagogicheskij vestnik. 2010; 2: 71–4 (in Russ.).
7. Golubeva M. G. Some aspects of the anti-stress action of tiroliberin and its synthetic analogue on erythrocytes [Nekotorye aspekty antistressogennogo dejstviya tiroliberina i ego sinteticheskogo analoga na eritrocity]. Tromboz, gemostaz i reologiya. 2012; 3: 48–51 (in Russ.).
8. Seydakhmetova Z. Zh., Tashenova G. K. Influence of immobilization stress on the reactivity of the sympathoadrenal system and erythrocyte resistance in rats during mammo- and lactogenesis [Vliyanie immobilizacionnogo stressa na reaktivnost’ simpatoadrenalovoj sistemy i rezistentnost’ eritrocitov u krys v period mammo- i laktogeneza]. Byulleten’ SO RAMN. 2005; 118 (4): 93–5 (in Russ.).
9. Roitman E. V., Morozov Yu. A., Isaeva A. M., Dementieva I. I. The phenomenon of adrenergic aggregation of erythrocytes in the experiment and in the clinic [Fenomen adrenergicheskoj agregacii eritrocitov v eksperimente i v klinike]. Tromboz, gemostaz i reologiya. 2004; 3 (19): 69–73 (in Russ.).
10. Shitikova A. S. Thrombocytic hemostasis [Trombocitarnyj gemostaz]. Saint-Petersburg: Izd-vo SPbGMU. 2000: 227 s (in Russ.).
11. Golubeva M. G., Grigorjeva M. Е. Influence of arginine-containing peptides on the haemostasis system. In: Arginine Amino Acid. N. Y., USA: Nova Science Publishers Inc. 2011: 229–35.