Тромбоз, гемостаз и реология

Tromboz, Gemostaz I Reologiya
scientific and practical journal

ISSN 2078–1008 (Print); ISSN 2687-1483 (online)
2021, Original papers
2021

Effect of new chalcone analogues on hemostasis in animals with experimental «сytokine storm»: 616-002:616.151.55

Original papers
Published 2021-10-04
Dmitry Pozdnyakov
Pyatigorsk Medical and Pharmaceutical Institute — Branch of Volgograd State Medical University, Health Ministry of Russian Federation; 11 Kalinin Avenue, Pyatigorsk 357532, Russia
Пятигорский медико-фармацевтический институт — филиал ФГБОУ ВО «Волгоградский государственный медицинский университет» Министерства здравоохранения Российской Федерации; Россия, 357500 Пятигорск, проспект Калинина, 11
Victoriya Rukovitsina
Pyatigorsk Medical and Pharmaceutical Institute — Branch of Volgograd State Medical University, Health Ministry of Russian Federation; 11 Kalinin Avenue, Pyatigorsk 357532, Russia
Пятигорский медико-фармацевтический институт — филиал ФГБОУ ВО «Волгоградский государственный медицинский университет» Министерства здравоохранения Российской Федерации; Россия, 357500 Пятигорск, проспект Калинина, 11
Anastasia Sosnovskaya
Pyatigorsk Medical and Pharmaceutical Institute — Branch of Volgograd State Medical University, Health Ministry of Russian Federation; 11 Kalinin Avenue, Pyatigorsk 357532, Russia
Пятигорский медико-фармацевтический институт — филиал ФГБОУ ВО «Волгоградский государственный медицинский университет» Министерства здравоохранения Российской Федерации; Россия, 357500 Пятигорск, проспект Калинина, 11
Elena Olokhova
Voino-Yasenetsky Krasnoyarsk State Medical University, Health Ministry of Russian Federation; 1 Partizana Zheleznyaka Str., Krasnoyarsk 660022, Russia
ФГБОУ ВО «Красноярский государственный медицинский университет имени профессора В.Ф. Войно-Ясенецкого» Министерства здравоохранения Российской Федерации; Россия, 660022 Красноярск, ул. Партизана Железняка, 1Партизана Железняка, 1

Keywords

chalcone analogues
«cytokine storm»
coagulopathy
hypercytokinemia
  Full Text

Abstract

Summary. Background. «Cytokine storm» is a disorder of the immune system with severe hypecytokinemia, characterized by the development of coagulation disorders with a high level of mortality. Objectives: to evaluate the effect of new chalcone analogues on changes of hemostasis reactions in rats under the conditions of an experimental «cytokine storm». Materials/Methods. The study was performed on 80 male Wistar rats divided into 8 equal groups of 10 individuals. The «cytokine storm» was modeled in animals by intraperitoneal injection of lipopolysaccharide at a dose of 10 mg/kg. The test-compounds at a dose of 20 mg/kg intraperitoneally and the reference drug — heparin (20 U/kg, subcutaneously) were administered 60 minutes after the pathology simulation. After 24 hours, the serum levels of fibrinogen, D-dimer, soluble fibrin-monomer complexes, antithrombin III activity, thrombin time, and the degree of ADP-stimulated platelet aggregation were evaluated in rats. Results. The study showed that the use of chalcone analogues contributed to the restoration of hemostasis reactions, which was expressed in a decrease in the concentration of fibrinogen, D-dimer, soluble fibrin-monomer complexes, the degree of platelet aggregation, and an increase in antithrombin III activity and thrombin time. At the same time, among the studied substances, the compound containing hydroxyl in the 2nd position and the methyl group in the 5th position showed a slightly higher level of pharmacological activity than the other test compounds. Conclusions. Based on the obtained data, it is actuality to assume the relevance of further study of chal- cone analogues as agents that normalize hemostasis in hypercytokine disorders.

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