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Abstract
Summary. Background. The deformation ability of erythrocytes is determined by the degree of hemoglobin hydration, membrane viscous properties, and the specific surface area of the red blood cell. The method of osmotic gradient ektacytometry has been successfully used for evaluation of erythrocyte functional properties. Objectives: to suggest additional evaluation parameters for erythrocyte rheological properties and methods for their measurement. Materials/Methods. Experiments were performed on erythrocytes from blood of 5 Wistar rats. Gradient ektacytometry on experimental laboratory unit was used to study erythrocytes deformation properties. Erythrocyte elongation index was studied. Impact of bifunctional sulfhydryl oxidizing reagent — diamide was used to increase the shear modulus of erythrocyte membrane. Whole blood diamide concentrations in the range of 0.01– 0.5 mmol/L were used. Results. Erythrocyte osmotic deformability profiles recorded at the high shear rate (τ=10.5 N/m2) begin to change markedly only with a diamide concentration of 0.1 mmol/L. At a low shear rate (τ=1.0 N/m2), decreasing of erythro- cyte membrane flexibility was detected by reduced deformation already at a diamide concentration of 0.02 mmol/L. Conclusions. Three new indices, namely the osmotic deformation range, passive water permeability, and membranes microviscosity, have been justified to add the range of certified capabilities of osmotic gradient ektacytometry method for assessing the determinants of erythrocyte deformation properties. Measurement at low shear rate allows to assess polymorphism of peripheral blood eryth- rocytes by morphological characteristics (anisocytosis). The proposed parameters significantly expand the diagnostic potential of the gradient ectacytometry method.
References
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