Effect of gasotransmitters and ozone on erythrocyte microrheology and blood oxygen transport function: 546.221.1:612.822:616

Abstract

Summary. Background. Erythrocyte microrheological characteristics are important for effective tissue perfusion, because the exchange capillaries have no sufficient dilatation reserves due to the absence of muscle elements. At the same time, erythrocytes can change their microrheology and oxygen transport function (OTF) under the influence of signaling molecules, such as gasotransmitters (GT) — nitric oxide (NO) and hydrogen sulfide (H2S). Other compounds, including ozone, can also modulate their effects. Objectives: comprehensive analysis of isolated effect of GT and ozone, as well as their combined action on erythrocyte microrheology and blood OTF in general. Materials/Methods. From 40 practically healthy men (mean age 27.1±1.4 years), 2 groups were formed based on the determination of maximum oxygen consumption (VO2max): group 1 — 20 men with moderate oxygen supply (VO2max < 50 ml/kg/min) and group 2—20 men with relatively high oxygen levels (VO2max>51 ml/kg/min). We registered skin oxygen tension (tсрО2) in the forearm, NO metabolism (according to the nitrate/nitrite ratio, NOx). We studied GT role in changes of erythrocyte microrheology; erythrocytes were incubated with GT donors alone and in combination with ozone, followed by registration of erythrocyte deforma- bility (DE) and aggregation (AE). In another series of experiments we registered OTF parameters of whole blood samples taken from 15 white male rats weighing 250–300 g. Preliminarily hypocapnic conditions were modeled, serum was incubated with ozone, with GT donors, and in combination with GT and ozone; then changes of OTF, NOx and H2S levels in serum were determined. Results. Persons with relatively high tissues supply with oxygen had more effective erythrocyte microrheology, which was indicated by positive changes in DE and AE both under the isolated effect of GT and ozone donors, and under their combined action. Another experi- mental model showed that blood incubation with ozonized physiological solution (ozone concentration — 2, 6, 10 mg/l) modified blood OTF, that manifested by increasing of oxygen tension, the degree of oxygenation and by decreasing of hemoglobin affinity for oxygen. Ozon action increases serum content of NO and H2S, which is important for modifying blood oxygen-binding properties. Conclusions. Ozone positively affects on both the most important microrheological characteristics of erythrocytes, on which their transport potential depends. Isolated use of GT donors increases DE and reduces AE. The combined action of GT and ozone slightly more increases erythrocyte microrheological answers to GT. Preliminary blood processing with hypocapnic gas mixture significantly increases ozone effect on blood OTF; the addition of nitroglycerin in these conditions further enhances ozone effect on blood OTF.

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