Тромбоз, гемостаз и реология

Tromboz, Gemostaz I Reologiya
scientific and practical journal

ISSN 2078–1008 (Print); ISSN 2687-1483 (online)
2023, Original papers
2023

Ontogenetic features of hemorheology and oxidative metabolism integration: 591.3:612.1

Original papers
Published 2024-04-03
Alexandra N. Popovicheva
Privolzhsky Research Medical University, Ministry of Health of the Russian Federation; 10/1 Minin and Pozharsky Square, Nizhny Novgorod 603005, Russia;
ФГБОУ ВО «Приволжский исследовательский медицинский университет» Министерства здравоохранения Российской Федерации; Россия, 603005 Нижний Новгород, площадь Минина и Пожарского, 10/1;
https://orcid.org/0000-0002-3717-2186 (unauthenticated)
Andrew K. Martusevich
Privolzhsky Research Medical University, Ministry of Health of the Russian Federation; 10/1 Minin and Pozharsky Square, Nizhny Novgorod 603005, Russia; Sechenov University; 8 bldg. 2, Trubetskaya Str., Moscow 119991, Russia
ФГБОУ ВО «Приволжский исследовательский медицинский университет» Министерства здравоохранения Российской Федерации; Россия, 603005 Нижний Новгород, площадь Минина и Пожарского, 10/1; ФГАОУ ВО Первый московский государственный медицинский университет имени И.М.Сеченова Министерства здравоохранения Российской Федерации (Сеченовский университет); Россия, 119991 Москва, ул. Трубецкая, 8, стр. 2
https://orcid.org/0000-0002-0818-5316 (unauthenticated)

Keywords

children
ontogenesis
hemorheology
oxidative blood metabolism
  Full Text

Abstract

Summary. Introduction. The microcirculatory system and blood rheology are essential for cellular and tissue metabolism. Free radical oxidation significantly affects hemorheology. An interesting field of study is the relationship between these blood properties and their effects at different ages of human ontogenesis. Aim: to investigate the age-related features of blood rheology and its oxidative metabolism in individuals of different ages, as well as the correlation between these factors. Materials and Methods. The study used blood samples from individuals considered to be almost healthy. The sample groups were categorized by age and gender, consisting of 30 participants aged up to 7 years, 34 aged 7–11 years, 32 aged 12–17 years, and 36 aged 18 years and older. We examined rheological properties, including erythrocyte deformability, aggregation, and disaggregation, as well as the state of their membranes and cytoskeleton. Additionally, we evaluated indicators of prooxidant and antioxidant blood metabolism, such as malondialdehyde level in plasma and erythrocytes, ischemia-modified albumin and plasma SH-groups, erythrocyte catalase activity, and glutathione concentration. Results. The ageing process correlates with a tendency towards more shear-induced and dextran-induced aggregation of erythrocytes. This manifests in various changes towards their disaggregation, showcasing disruptions in the cytoskeleton stability, and a decreased deformability tendency. Plasma malondialdehyde level does not change (in contrast to ischemia-modified albumin). However, erythrocytes exhibit multidirectional changes with respect to malondialdehyde level. The catalase activity increased, the concentration of glutathione and SH-groups showed a similar trend. Significant correlations of varying strengths and directions were discovered between the rheological, prooxidant, and antioxidant properties of blood in healthy individuals older than 7 years. Conclusion. Significant changes in hemorheological properties occur during different stages of ontogenesis. At the same time, the prooxidant and antioxidant systems undergo a transformation, leading to gradual activation of free radical processes in plasma and erythrocytes. This, in turn, is compensated by the stimulation of their antioxidant potential. Additionally, the process of growth and development is characterized by the gradual formation and complication of links between the studied blood properties.

For citation: Popovicheva A.N., Martusevich A.K. Ontogenetic features of hemorheology and oxidative metabolism integration. Tromboz, gemostaz i reologiya. 2023;(4):59–67. (In Russ.).

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