Experimental models of parenchymal bleeding for assessing the efficacy of local hemostatic agents: 616-001.41

Abstract

Summary. Introduction. Marketing new medical materials, including local hemostatic agents (LHA), necessitates in vivo experiments, in which various methods are used to determine the efficacy of LHA in heavy bleeding models. Standardization and ease of reproducibility are requirements for these models. Aim: to consider various rat parenchymal bleeding models as well as methods for assessment of LHA efficacy.
Materials and Methods. The search for scientific publications was conducted out in the Google Scholar and PubMed databases over the past 15 years. Search queries included the following keywords both in Russian and English: “parenchymal bleeding”, “experimental rat bleeding model”, and “local hemostatic agents”. The inclusion criteria were as follows: the article must be a full-text article with original results and must concern a parenchymal hemorrhage model in rats. The exclusion criteria were as follows: the article must not be a litera ture review, it must not concern a parenchymal hemorrhage model in other animals and hemorrhagic shock model (the one assessing systemic hemody- namic parameters). Based on 38 literature sources, an analysis of parenchymal bleeding models, as well as methods for LHA assessing both in vivo and in vitro, was carried out. Results. Various models of parenchymal bleeding and methods for assessing LHA efficacy have been reviewed. With regard to the potential for reproducing experimental research, the majority of articles indicate a lack of systematic and stan- dardized methodology in existing models. Conclusion. The considered aspects of parenchymal bleeding modeling in rats can help create an up-to-date model for investigating the original LHA and comparing them with each other.

For citation: Shabunin A. S., Rodionova K. N., Safonov P. A., Novosad Yu.A., Vissarionov S. V. Experimental models of parenchymal bleeding for assessing the efficacy of local hemostatic agents. Tromboz, gemostaz i reologiya. 2024;(4):12–19. (In Russ.).

References

1. Ko Y. G., Kim B. N., Kim E. J. et al. Bioabsorbable carboxymethyl starch–calcium ionic assembly powder as a hemostatic agent. Polymers (Basel). 2022;14(18):3909. DOI: 10.3390/polym14183909.
2. Landi M., Everitt J., Berridge B. Bioethical, reproducibility, and translational challenges of animal models. ILAR J. 2021;62(1–2):60–5. DOI: 10.1093/ilar/ilaa027.
3. Kinzersky A. A., Dolgikh V. T., Korzhuk M. S. et al. The Wistar rat line hemostatic system characteristics to be important for experimental surgery. Vestnik eksperimental’noj i klinicheskoj hirurgii. 2018;11(2):126–33. (In Russ.). DOI: 10.18499/2070-478X-2018-11- 2-126-133.
4. Siller-M atula J. M., Plasenzotti R., Spiel A. et al. Interspecies differences in coagulation profile. Thromb Haemost. 2008;100(3):397– 404.
5. Morgan C. E., Prakash V. S., Vercammen J. M. et al. Development and validation of 4 different rat models of uncontrolled hemorrhage. JAMA Surg. 2015;150(4):316–24. DOI: 10.1001/jamasurg.2014.1685.
6. Kochan J., Schmidtová L., Sadloňová I. et al. Hemostatic effect and distribution of new rhThrombin formulations in rats. Interdiscip Toxicol. 2014;7(4):219–22. DOI: 10.2478/intox-2014-0032.
7. Aydin O., Tunkal S., Kilicoglu B. et al. Effects of Ankaferd Blood Stopper and calcium alginate in experimental model of hepati parenchymal bleeding. Bratisl Lek Listy. 2015;116(2):128–31. DOI: 10.4149/bll_2015_025.
8. Ibne Mahbub M. S., Bae S. H., Gwon J.-G., Lee B.-T. Decellularized liver extracellular matrix and thrombin loaded biodegradable TOCN/Chitosan nanocomposite for hemostasis and wound healing in rat liver hemorrhage model. Int J Biol Macromol. 2023;225:1529– 42. DOI: 10.1016/j.ijbiomac.2022.11.209.
9. Lei C., Zhu H., Li J. et al. Preparation and hemostatic property of low molecular weight silk fibroin. J Biomater Sci Polym Ed. 2016;27(5):403–18. DOI: 10.1080/09205063.2015.1136918.
10. Gu R., Sun W., Zhou H. et al. The performance of a fly-larva shellderived chitosan sponge as an absorbable surgical hemostatic agent. Biomaterials. 2010;31(6):1270–7. DOI: 10.1016/j.biomateri- als.2009.10.023.
11. Aysan E., Bektas H. Ercoz F. et al. Ability of the ankaferd blood stopper® to prevent parenchymal bleeding in an experimental hepatic trauma model. Int J Clin Exp Med. 2010;3(3):186–91.
12. Girish A., Hickman D. A., Banerjee A. et al. Trauma-targeted delivery of tranexamic acid improves hemostasis and survival in rat liver hemorrhage model. J Thromb Haemost. 2019;17(10):1632–44. DOI: 10.1111/jth.14552.
13. Jaiswal A. K., Chhabra H., Narwane S. et al. Hemostatic efficacy of nanofibrous matrix in rat liver injury model. Surg Innov. 2017;24(1):23–8. DOI: 10.1177/1553350616675799.
14. Satar N. Y.G., Akkoc A., Oktay A. et al. Evaluation of the hemostatic and histopathological effects of Ankaferd Blood Stopper in experimental liver injury in rats. Blood Coagul Fibrinolysis. 2013;24(5):518–24. DOI: 10.1097/MBC.0b013e32835e9498.
15. Komachi T., Sumiyoshi H., Inagaki Y. et al. Adhesive and robust multilayered poly (lactic acid) nanosheets for hemostatic dressing in liver injury model. J Biomed Mater Res Part B Appl Bioma- ter. 2017;105(7):1747–57. DOI: 10.1002/jbm.b.33714.
16. Wakabayashi T., Yagi H., Kitagawa Y. et al. Efficacy of new polylactic acid nonwoven fabric as a hemostatic agent in a rat liver resection model. Surg Innov. 2019;26(3):312–20. DOI: 10.1177/1553350619833582.
17. Gurevich K. G., Urakov A. L., Bashirova L. I. et al. The hemostatic activity of bis (2-aminoethan-1-sulfonate) calcium. Asian J Pharm Clin Res. 2018;11(11):452–5. DOI: 10.22159/ajpcr.2018.v11i11.29049.
18. Muench T. R., Kong W., Harmon A. M. The performance of a hemostatic agent based on oxidized regenerated cellulose — polyglactin 910 composite in a liver defect model in immunocompetent and athymic rats. Biomaterials. 2010;31(13):3649–56. DOI: 10.1016/j. biomaterials.2010.01.070.
19. Zhu J., Wu Z., Sun W. et al. Hemostatic efficacy and biocompatibility evaluation of a novel absorbable porous starch Hemostat. Surg Innov. 2022;29(3):367–77. DOI: 10.1177/15533506211046100.
20. Wang D., Li W., Wamg Y. et al. Fabrication of an expandable keratin sponge for improved hemostasis in a penetrating trauma. Colloids Surf B Biointerfaces. 2019;182:110367. DOI: 10.1016/j.col- surfb.2019.110367.
21. Wang J., Hao S., Luo T. et al. Development of feather keratin nanoparticles and investigation of their hemostatic efficacy. Mater Sci Eng C Mater Biol Appl. 2016;68:768–73. DOI: 10.1016/j. msec.2016.07.035.
22. Nouri S., Sharif M. R. Efficacy and safety of ferric chloride in controlling hepatic bleeding; an animal model study. Hepat Mon. 2014;14(6): e18652 DOI: 10.5812/hepatmon.18652.
23. Chen J., Ai J., Chen S. et al. Synergistic enhancement of hemostatic performance of mesoporous silica by hydrocaffeic acid and chitosan. Int J Biol Macromol. 2019;139:1203–11. DOI: 10.1016/j. ijbiomac.2019.08.091.
24. Goncharuk O., Korotych O., Samchenko Y. et al. Hemostatic dressings based on poly (vinyl formal) sponges. Mater Sci Eng C Mater Biol Appl. 2021;129:112363. DOI: 10.1016/j.msec.2021.112363.
25. Şener D., Kocak M., Saracoglu R. et al. Histopathological effects of Algan hemostatic agent (AHA) in liver injury model in rats. Hepatol Forum. 2021;3(1):16–20. DOI: 10.14744/hf.2021.2021.0040.
26. Wang Y., Xiao D., Zhong Y. et al. Preparation and characterization of carboxymethylated cotton fabrics as hemostatic wound dressing. Int J Biol Macromol. 2020;160:18–25. DOI: 10.1016/j. ijbiomac.2020.05.099.
27. Panwar V., Thomas J., Sharma A. et al. In-vitro and in-vivo evaluation of modified sodium starch glycolate for exploring its haemostatic potential. Carbohydr Polym. 2020;235:115975. DOI: 10.1016/j.carbpol.2020.115975.
28. Ferretti L., Qiu X., Villalta J., Lin G. Efficacy of BloodSTOP iX, surgicel, and gelfoam in rat models of active bleeding from partial nephrectomy and aortic needle injury. Urology. 2012;80(5):1161.e1–6. DOI: 10.1016/j.urology.2012.06.048.
29. Song H., Zhang L., Zhao X. Hemostatic efficacy of biological selfassembling peptide nanofibers in a rat kidney model. Macromol Biosci. 2010;10(1):33–9. DOI: 10.1002/mabi.200900129.
30. Huri E., Akgül T., Ayyildiz A. et al. Hemostatic role of a folkloric medicinal plant extract in a rat partial nephrectomy model: controlled experimental trial. J Urol. 2009;181(5):2349–54. DOI: 10.1016/j.juro.2009.01.016.
31. Midi A., Ekici H., Kumandas A. et al. Investigation of the effec- tiveness of algan hemostatic agent in bleeding control using an experimental partial splenectomy model in rats. Marmara Med J. 2019;32(1):27–32. DOI: 10.5472/marumj.518821.
32. Portilla- De- Buen E., Ramos L., Leal C. et al. Activated clotting time and heparin administration in sprague — d awley rats and syri an golden hamsters. Contemp Top Lab Anim Sci. 2004;43(2):21–4.
33. Deineka V., Sulaieva O., Pernakov M. et al. Hemostatic and tissue regeneration performance of novel electrospun chitosan-based materials. Biomedicines. 2021;9(6):588. DOI: 10.3390/biomedicines9060588.
34. Ibne Mahbub M. S., Sultana T., Gwon J.-G., Leev B.-T. Fabrication of thrombin loaded TEMPO-oxidized cellulose nanofiber-gelatin sponges and their hemostatic behavior in rat liver hemorrhage model. J Biomater Sci Polym Ed. 2022;33(4): 499–516. DOI: 10.1080/09205063.2021.1992877.
35. Adams G. L., Manson R. J., Hasselblad V. et al. Acute in-vivo evaluation of bleeding with GelfoamTM plus saline and Gel- foam plus human thrombin using a liver square lesion model in swine. J Thromb Thrombolysis. 2009;28(1):15. DOI: 10.1007/s11239-008-0249-3.
36. Lewis K. M., Atlee H. D., Mannone A. J. et al. Comparison of two gelatin and thrombin combination hemostats in a porcine liver abrasion model. J Investig Surg. 2013;26(3):141–8. DOI: 10.3109/0 8941939.2012.724519.
37. Dasgupta N., Ranjan S., Shree M. et al. Blood coagulating effect of marigold (Tagetes erecta L.) leaf and its bioactive com- pounds. Orient Pharm Exp Med. 2016;16:67–75. DOI: 10.1007/ s13596-015-0200-z.
38. Ikese C. O., Okoye Z. C., Kukwa D. T. et al. Effect of aqueous leaf extract of Tridax procumbens on blood coagulation. Int J Pharm Sci Res. 2015;6(8):3391–5. D