The impact of a single physical load on age-dependent proteins level, hemostasis and the microcirculatory bed of aged animals

Abstract

Summary. Introduction. Physical exercise, depending on its duration, intensity, and frequency, may exhibit both adaptive and maladap‐ tive effects on the body. The state of the body is largely determined by the balance between important age‐dependent proteins. Currently, the literature contains no convincing data on non‐drug management of irisin and chemokine CCL11 levels using an optimal regimen of physical activity. Aim: to identify the effects exerted on aged rats by a single 2‐hour physical load by determining the state of hemostasis and microcirculatory bed, as well as the level of irisin and CCL11 proteins. Materials and Methods. All animals participating in the study (Wistar aged male rats, n = 24) were divided into two groups — experimental (n = 12) and control (n = 12). The experimental group was subjected to a single 2‐hour treadmill walk, the control group was not subjected to physical activity. We assessed the level of irisin and CCL11 proteins in blood serum and in cardiac and femoral muscles, as well as the primary indicators of hemodynamics, coagulation profile and thrombo‐ elastography parameters. Results. In aged animals, a single 2‐hour physical load resulted in an increase in irisin levels in the blood serum and a decrease in CCL11 concentrations in the heart muscle. The hemodynamic balance of the velocity distribution shifted towards the

center of vascular shifts. The hypercoagulation phenomena were accompanied by a decrease in anticoagulant activity. Correlations between CCL11 level and the main hemodynamic parameters (HI 3, PULSE 2) were found. There were also negative correlations between the content of irisin in the femoral muscle and the number of platelets, as well as a positive relationship between the level of irisin in blood serum and fibrinolytic activity. There was a positive relationship between serum CCL11 concentration and prothrombin time. CCL11 level in the heart muscle had a negative relationship with the index of maximum clot density. Conclusion. The findings indicate that a single 2‐hour exercise session can influence blood level of irisin and CCL11, with these proteins playing a role in regulating blood flow and clotting in aged animals.

For citation: Bondarchuk Yu.A., Manaeva I.N., Momot A.P., Shakhmatov I.I. The impact of a single physical load on age‐dependent proteins level, hemostasis and the microcirculatory bed of aged animals. Tromboz, gemostaz i reologiya. 2025;(4):12–20. (In Russ.).

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